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Tutorial RNASeq for detection of pre-tumoral biomarkers using a group case study of Proliferative Verrucous Leukoplakia
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RNASeq Leukoplakia
PATHseq
CONTENTS
1. PRELIMINARY INFORMATION
1.1 - Tutorial objective
1.2 - Tutorial material and case study
1.3 - Experiment design and support
1.4 - Installing and activating RNASeq and the Server-Side
2. STEP-BY-STEP MODE TUTORIAL
2.1. - TOPHAT/HISAT2 & CUFFLINKS
2.1.1 - Preparing your experiment
2.1.2 - Quality analysis and preprocessing
2.1.3 - Mapping
2.1.4 - Transcriptome Assembly
2.1.5 - Differential Expression analysis
2.1.6 - GOSeq
3. PIPELINE MODE TUTORIAL
3.1 - TopHat/Hisat2 & Cufflinks protocol
4. ACKNOWLEDGEMENTS
5. BIBLIOGRAPHY
CITE US
PATHseq
diff_genes_Biased_Data.pdf
diff_genes_GO.enrichment.samp.csv
diff_genes_GO.enrichment.wall.csv
diff_genes_GO.samp.csv
diff_genes_GO.wall.csv
diff_genes_pwf_fit.pdf
diff_genes_pwf.csv
diff_genes_WALLvsNOBIAS.pdf
diff_genes_WALLvsSAMP.pdf
goseq_log
Download folder
PATHseq
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Course sections
CONTENTS
1. PRELIMINARY INFORMATION
1.1 - Tutorial objective
1.2 - Tutorial material and case study
1.3 - Experiment design and support
1.4 - Installing and activating RNASeq and the Server-Side
2. STEP-BY-STEP MODE TUTORIAL
2.1. - TOPHAT/HISAT2 & CUFFLINKS
2.1.1 - Preparing your experiment
2.1.2 - Quality analysis and preprocessing
2.1.3 - Mapping
2.1.4 - Transcriptome Assembly
2.1.5 - Differential Expression analysis
2.1.6 - GOSeq
3. PIPELINE MODE TUTORIAL
3.1 - TopHat/Hisat2 & Cufflinks protocol
4. ACKNOWLEDGEMENTS
Pipeline mode: Tophat & Cufflinks protocol results
5. BIBLIOGRAPHY
CITE US
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Biotechvana
Valencia Lab
Parc Cientific Universitat de Valencia
Carrer del Catedràtic Agustín Escardino, 9. 46980 Paterna (Valencia) Spain
Madrid Lab
Parque Científico de Madrid
Campus de Cantoblanco
Calle Faraday 7, 28049 Madrid Spain
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biotechvana@biotechvana.com
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