FastQCFastQC Report
Tue 15 Feb 2022
CAN4_R1_all.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAN4_R1_all.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15433392
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG116300.08.2541071
AGGGGGG59150.06.97207931
TTTTCGC13800.008172735.123748370
GCGAACT10856.491236E-45.109983423
Produced by FastQC (version 0.11.5)