FastQCFastQC Report
Fri 14 Jan 2022
ZFG-17-12_20_26350_S6_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_20_26350_S6_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18319281
Sequences flagged as poor quality0
Sequence length45-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGAGTGCTTCTGGCTCAGCTTGGTGTAAGCATTCTTGATGTCGTCCA241000.13155538145847537No Hit
GTGGAGTTTAGCGGTATCTCTCGGCCAGAGCCAGAGCCAAGTTCTGCAGGA235170.12837294214767492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA59250.023.31233643
TCCTACT61550.022.66179842
ATTCCTA62600.022.18822740
TTCCTAC65700.021.28382541
ATATTCC71850.019.45444138
GCCAATT87150.017.957011
GCTTATT51300.017.425681
ATACGAT22200.017.41127637
GAGTTAA82650.017.2379717
TATTCCT83250.016.87125239
GCGGTAT67750.016.65144311
TTTAGCG77100.016.4998517
TGAGTTA86200.016.47609516
TTAGCGG70750.016.3917228
GATACGA24050.016.35200336
GTTCGAA14400.016.1061361
CGCTACG9250.016.03430736
CATATTC89000.015.90763837
GTTAAAC90650.015.51841719
AGAGTGC82450.015.3739985
Produced by FastQC (version 0.11.5)