FastQCFastQC Report
Fri 14 Jan 2022
ZFG-17-12_12_26342_S16_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_12_26342_S16_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21532879
Sequences flagged as poor quality0
Sequence length45-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA24850.015.68262143
CGTTCGA13300.013.51082445
TCCTACT31350.012.91951342
TTCCTAC33250.012.31130941
CGCAAGG17100.010.9124436
CCCGAAT11350.010.8966111
GCCCTAT5400.010.8269021
CCAATTG27100.010.5374083
ATTCCTA36950.010.46732340
CGAGATA33100.010.34461943
CCGCAAG17550.010.24832235
ATATTCC40950.010.21198238
GTCGCTA15350.010.10661727
CGTTCCG35000.010.085155538
AGATAGG35450.010.0079145
TCGCTAT15100.09.97603428
CTAGATA21700.09.94706117
GCCAATT51350.09.7215861
GTTCCGA35050.09.68607839
CCGTGAT22300.09.5795111
Produced by FastQC (version 0.11.5)