FastQCFastQC Report
Wed 5 Jan 2022
ZFG-17-12_32_26362_S18_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_32_26362_S18_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18838425
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGTTTAGCGGTATCTCTCGGCCAGAGCCAGAGCCAAGTTCTGCAGGA196010.1040479764099175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA52550.017.50675643
TCCTACT55500.016.7387342
TTCCTAC56800.016.39474941
ATTCCTA57000.016.25808540
GCCAATT73450.015.0412711
CGCTACG12050.014.93278336
ATATTCC65900.014.13030738
TCCGCTA12450.014.09221334
AATCCGC12700.013.8145532
GCGGTAT68000.013.66183211
GCTACGC13500.013.32899737
CGCTTAA12500.013.31602141
GTCCATT77300.013.2129271
TTTAGCG80350.013.1885947
TTAGCGG73600.013.144638
GCCTACT11000.012.913031
GTCACAT90800.012.5396621
CCAATTT82600.012.4166092
GAATCCG14050.012.32770331
CGGCACC92650.012.3107923
Produced by FastQC (version 0.11.5)