FastQCFastQC Report
Wed 5 Jan 2022
ZFG-17-12_28_26358_S14_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_28_26358_S14_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18276109
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA30000.016.9453843
TTCCTAC35300.014.84734741
TCCTACT34600.014.82299342
ATTCCTA35950.013.89028240
GCCAATT49850.013.7044421
CGCTACG9250.012.158812536
AAGCACG24600.011.71881445
ATATTCC46700.011.0298238
GCTTCGC33600.010.85886845
TTAGCGG38050.010.820418
GCGGTAT36050.010.54537811
GTCGCTT22550.010.1985281
GAGTTAA57450.010.17954517
TGCTTCG37250.010.08375344
TTTAGCG46450.010.0747817
CCAATTT61200.010.016822
GTTAAAC59050.09.94222319
GTCCGTT35350.09.8223461
TGAGTTA58200.09.7778616
TCCGATT65800.09.74315726
Produced by FastQC (version 0.11.5)