FastQCFastQC Report
Wed 5 Jan 2022
ZFG-17-12_16_26346_S2_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_16_26346_S2_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20058031
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA26400.013.80238143
CGCTACG11350.011.49450436
GCCAATT45200.011.4737021
TCCTACT32600.011.38491642
GCGGTAT44200.011.24714211
TCCGCTA11800.011.24688934
CGCTTAA12550.010.93295941
TTTAGCG57850.010.8118297
TTAGCGG50300.010.6005228
TTCCTAC37050.010.38148541
GTCCGAT41300.010.1549331
GTGCATT69600.010.0430741
GTCGCGT11900.010.0425291
AATCCGC12350.010.01719832
GTCGGTT25250.010.0016311
GCTACGC13000.09.86250937
ATACGAT17350.09.85303537
CGGTATC58400.09.70592912
GGGGATT53400.09.5429221
AAGCACG32050.09.27561145
Produced by FastQC (version 0.11.5)