FastQCFastQC Report
Wed 5 Jan 2022
ZFG-17-12_12_26342_S16_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_12_26342_S16_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22034675
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA13750.013.73886745
CCTACTA26950.012.18348143
GCCGCAA18400.011.12566734
TCGGATT15000.011.1014822
CCGCAAG18850.011.09771835
CGCAAGG18500.010.69900636
CTCGGAT19700.010.1662631
TCCTACT34550.09.89413442
GTCGCTA16450.09.85003427
GTCGGAT24600.09.7878261
CCGGAAA11450.09.6300421
TTCCTAC36700.09.49836541
CCCGGAT32250.09.41981
CTCCTAT30100.09.4197991
CGCTAGT13100.09.27044944
GTCCTAT16350.09.2213531
ATTCGAA18800.09.0963618
CCAATTG28800.09.0633193
GCCGAAT9750.09.0011421
CGATTGA17800.08.9753274
Produced by FastQC (version 0.11.5)