FastQCFastQC Report
Wed 5 Jan 2022
ZFG-17-12_06_26336_S10_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_06_26336_S10_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15232865
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCTA45400.020.3583440
TCCTACT46800.019.7982942
TTCCTAC47700.019.42467541
CCTACTA47900.019.06167843
ATATTCC58250.016.44739538
GAGTTAA60950.015.8042717
TATTCCT61350.015.57960439
GTTAAAC64750.014.6334519
TGAGTTA66050.014.41367716
GCCAATT70200.014.1993451
CATATTC70250.013.99076337
AAGCACG32350.013.06931345
ATGAGTT74950.012.85238415
AACATAT74300.012.714835
CCCGAAT7050.012.4473571
CCCGGAT26100.011.9832861
CAATTTG85100.011.8190483
GTGCATT56450.011.7188521
AATTTGA98300.011.6284124
ACATATT84200.011.45957836
Produced by FastQC (version 0.11.5)