FastQCFastQC Report
Tue 18 Jan 2022
ZFG-17-12_32_26362_S18_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_32_26362_S18_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18000836
Sequences flagged as poor quality0
Sequence length45-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGTTTAGCGGTATCTCTCGGCCAGAGCCAGAGCCAAGTTCTGCAGGA189640.10535066260255913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA50350.018.39184243
TCCTACT52300.017.72243142
ATTCCTA54750.017.28347440
TTCCTAC56450.016.60818541
GCCAATT72650.015.1547161
ATATTCC63950.014.82982938
TTAGCGG69250.014.21521958
CGCTTAA9700.014.13865941
GCGGTAT67950.014.08913711
TTTAGCG75850.014.0748747
GGGGATT51800.013.5612391
GAGTTAA68750.013.27087317
CCAATTT76600.013.1393122
TGAGTTA69900.013.02053516
CGGTATC77150.013.02034712
TATTCCT73300.012.96886339
AAGCACG32550.012.84331845
GTGCATT70650.012.7474221
GTCACAT83900.012.7201121
GTTCGAT25200.012.6870991
Produced by FastQC (version 0.11.5)