FastQCFastQC Report
Tue 18 Jan 2022
ZFG-17-12_28_26358_S14_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_28_26358_S14_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17762078
Sequences flagged as poor quality0
Sequence length45-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA27750.019.31520343
TCCTACT34200.015.71956342
ATTCCTA35750.015.40499140
TTCCTAC36500.014.65991141
GCCAATT51850.013.1623761
GCGGTAT38700.011.673511511
ATATTCC46750.011.63499138
TTAGCGG40600.011.5162528
CCCGAAT9150.011.3234091
AATCCGC10750.011.29067932
GAGTTAA53750.010.9971217
CGCTACG10300.010.91085936
GCTTCGC33150.010.80489345
TTTAGCG49050.010.7242427
CCAATTT58050.010.7093832
TATTCCT53100.010.66693239
TGAGTTA54550.010.63001816
GTTAAAC56700.010.42501919
GTCCGAT33050.010.1544241
TACTACT62000.010.06339245
Produced by FastQC (version 0.11.5)