FastQCFastQC Report
Tue 18 Jan 2022
ZFG-17-12_24_26354_S10_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_24_26354_S10_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28070496
Sequences flagged as poor quality0
Sequence length45-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA48600.018.8122943
TCCTACT57200.016.20102942
ATTCCTA58550.016.0483940
TTCCTAC60300.015.28699641
GCCAATT83350.013.8901551
ATATTCC73050.012.98453338
TATTCCT80550.011.71975839
GAGTTAA88300.011.09775517
GTTAAAC90200.010.93893619
CCAATTT97250.010.9094042
TGAGTTA91350.010.70273416
GCGGTAT68500.010.53272711
TTTAGCG84000.010.4635077
TTAGCGG77050.010.4442638
CCCGAAT12250.010.2967821
GTCGATT25550.09.7854691
GTCCGAT52200.09.6655621
CATATTC102150.09.65962637
GGGGATT74900.09.5630161
TACTACT106850.09.55773145
Produced by FastQC (version 0.11.5)