FastQCFastQC Report
Tue 18 Jan 2022
ZFG-17-12_16_26346_S2_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_16_26346_S2_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19463442
Sequences flagged as poor quality0
Sequence length45-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA22200.015.21620943
GTGCATT69450.011.026071
GCGGTAT41050.010.95069911
GCCAATT46550.010.8378311
TCCTACT31350.010.83495642
TTAGCGG47650.010.4257388
TTTAGCG57900.010.1722987
CCCGAAT9600.010.0881441
TTCCTAC34250.010.04363441
GTCGGTT26950.09.94494151
GTCCGAT37250.09.8554251
GGGGATT52250.09.8279421
CGCCGAA23700.09.67334711
CCCGTAT10850.09.3410741
CGGTATC55250.09.31578112
GTCCATT74200.09.197141
GTCGATT21200.09.1364331
GTCGGAT23950.09.1217941
AAGCACG34500.09.03984245
GTCGCTT24950.09.0270011
Produced by FastQC (version 0.11.5)