FastQCFastQC Report
Tue 18 Jan 2022
ZFG-17-12_09_26339_S13_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_09_26339_S13_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16514775
Sequences flagged as poor quality0
Sequence length45-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA9700.010.70986845
GTCCGAT30450.09.9684171
GTGCATT52600.09.9170381
TTAGCGG33000.09.8778578
GCGGTAT31300.09.84030511
GTCCGTT29050.09.5974371
GTCCATT65800.09.5677951
GTCCTAT12450.09.3910411
CCGCAAG10850.09.32376835
GTCGAAT14000.09.3149321
TTTAGCG39750.09.2750327
GTCCGCT21150.08.9299411
CGCTACG9400.08.60960736
AATCCGC9950.08.58557232
CTCGTAT27600.08.5538161
GTCGGAT19800.08.5167871
CGCAAGG11200.08.43024136
GTCGATT15750.08.4226961
CCGATCG6451.8189894E-118.3659033
CTCCTAT19900.08.3610021
Produced by FastQC (version 0.11.5)