FastQCFastQC Report
Tue 18 Jan 2022
ZFG-17-12_06_26336_S10_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_06_26336_S10_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14847317
Sequences flagged as poor quality0
Sequence length45-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCTA45850.020.49877540
TCCTACT45850.020.26756942
TTCCTAC47050.019.64679341
CCTACTA48100.019.25034143
ATATTCC55400.017.1655338
TATTCCT59000.015.96599439
GTTAAAC64050.014.98907619
GAGTTAA63550.014.9302617
AAGCACG31550.014.62092145
TGAGTTA65500.014.52009916
GCCAATT70550.014.3423281
CATATTC70050.013.80015737
CCCGGAT25350.013.0389771
AACATAT73900.012.92909635
ATGAGTT76000.012.543447515
GTGCATT52550.012.3661
CCAATTT83900.011.8458012
CAATTTG85550.011.5642583
AATTTGA95450.011.4945624
ATTTGGC84450.011.4205925
Produced by FastQC (version 0.11.5)