FastQCFastQC Report
Tue 18 Jan 2022
ZFG-17-12_03_26333_S7_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_03_26333_S7_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16865336
Sequences flagged as poor quality0
Sequence length45-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA44650.018.56759643
TCCTACT47650.017.51795442
TTCCTAC48650.017.19723141
ATTCCTA49050.016.77804440
CGTTCGA11750.016.6797545
CGCAAGG12750.015.69303536
ATATTCC56150.015.13476438
ACCCGGG15300.013.51934827
CCGCAAG14750.013.41274935
TATTCCT63900.013.33466939
GCCAATT71200.013.26413251
GAGTTAA62400.013.2599917
GTTAAAC65900.013.10156519
GCCGCAA15100.012.95297234
TGAGTTA63500.012.8887716
CATATTC69200.012.47533737
AAGCACG31100.012.01055645
GATACGA22550.011.76420636
GCTCCGA73800.011.45495524
ATGAGTT74100.011.28781415
Produced by FastQC (version 0.11.5)