FastQCFastQC Report
Tue 18 Jan 2022
ZFG-17-12_24_26354_S10_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_24_26354_S10_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28812309
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA49050.018.57235543
TCCTACT56950.016.03619642
ATTCCTA60100.014.8959440
TTCCTAC61400.014.80048841
GCCAATT84050.013.0370881
ATATTCC74450.012.29658338
TATTCCT83350.011.19938639
GAGTTAA93200.010.69139317
TGAGTTA92350.010.59492916
GCGGTAT69550.010.47913111
CCAATTT97950.010.3550242
GTTAAAC96000.010.26253319
AAGCACG48350.010.24862645
TTTAGCG86600.010.2099517
TTAGCGG79400.010.115428
GGGGATT84100.09.8122191
GTCGATT28050.09.5652471
CATATTC101150.09.56230537
GTCGGTT37700.09.449261
TCGCTAG12350.09.28907142
Produced by FastQC (version 0.11.5)