FastQCFastQC Report
Tue 18 Jan 2022
ZFG-17-12_20_26350_S6_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_20_26350_S6_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19171471
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGAGTGCTTCTGGCTCAGCTTGGTGTAAGCATTCTTGATGTCGTCCA247280.1289833211024861No Hit
GTGGAGTTTAGCGGTATCTCTCGGCCAGAGCCAGAGCCAAGTTCTGCAGGA244340.1274497924546322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA56900.022.8492343
TCCTACT60700.021.38291542
ATTCCTA61650.021.01632540
TTCCTAC63100.020.85410741
ATATTCC71500.018.30968538
GCTTATT57100.017.689031
GCGGTAT70150.016.83436411
GTCCATT87500.016.773941
TTAGCGG71500.016.6450888
GCCAATT93950.016.3422741
TTTAGCG80800.016.1496987
GAGTTAA84700.016.06632817
CTTATGA46300.016.03179618
TGAGTTA84750.015.76474116
ATACGAT22550.015.66099337
CGGTATC76250.015.42796912
GTTCGAT24750.015.3947861
AGAGTGC85400.015.3331185
GTTAAAC87600.015.27784419
GTTTATT108850.015.2444421
Produced by FastQC (version 0.11.5)