FastQCFastQC Report
Tue 18 Jan 2022
ZFG-17-12_09_26339_S13_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_09_26339_S13_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16993867
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT7450.013.5927241
CGTTCGA9500.09.70581145
GTGCATT54700.09.626731
GGGGATT42900.09.3895761
TTAGCGG34500.09.2614158
AAGCACG25550.08.97802545
TTTAGCG40350.08.9223537
CTCCAAT58050.08.7610571
GTCCGAT30700.08.7228591
GTCCATT63950.08.6917391
CTCGTAT27450.08.60791
GTCGGTA8900.08.5968471
GCGGTAT31400.08.52842511
CGCAAGG12450.08.49122536
CTCGGAT14150.08.4288771
GTCGAAT14700.08.4196811
GTCCTAT12300.08.4159471
TCGAGTT17950.08.399592
CTCCTAT20100.08.39683151
GTCCGTT30250.08.3318781
Produced by FastQC (version 0.11.5)