FastQCFastQC Report
Wed 5 Jan 2022
ZFG-17-12_03_26333_S7_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameZFG-17-12_03_26333_S7_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17311182
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTA45950.018.69550943
TCCTACT47500.018.2749342
TTCCTAC48950.017.5498741
ATTCCTA51300.016.78889340
ATATTCC57300.015.1106538
CGTTCGA11650.014.86343945
GAGTTAA62300.013.90808217
GCCGCAA13550.013.61388634
CCGCAAG13100.013.56490935
GTTAAAC66150.013.23482219
TATTCCT66200.013.04488539
TGAGTTA65800.012.82655516
GATACGA23650.012.74374736
CGCAAGG13350.012.6358236
GCCAATT74300.012.6286351
ATACGAT24850.012.21815837
CATATTC73050.012.06880337
AAGCACG34950.011.13149245
AACATAT80000.010.96568435
CAATTTG84550.010.85827353
Produced by FastQC (version 0.11.5)