FastQCFastQC Report
lun 21 ene 2019
JVB-R1_S1_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R1_S1_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13465576
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT839910.6237460618097584No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206670.15348025216299696No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTT152430.11319976211934789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT13450.019.4066828
TCGACCG13650.019.37542327
GTTCGAC14300.018.98065225
CGCCCTT20450.016.6691191
AAGTTCG17500.015.5090423
CGCGTAA16750.014.95194151
ACGGTTA47000.014.8014953
CCGTTAA49750.014.40731621
GACCGTC18500.014.29772729
GCGTAAC18950.014.1336172
GCCGTTA50000.014.12640920
GGTACCG51400.014.07602811
CTAGAGG75950.013.95331270
GTTATCG17450.013.36540134
TCGTTCG17300.013.27730928
TTGTCGC40450.013.07486565
GGCCGTT55200.013.0468719
CACGGTT56800.012.8600532
GCGACGT19550.012.81491322
AAATCGC19800.012.64944354
Produced by FastQC (version 0.11.5)