FastQCFastQC Report
lun 21 ene 2019
JVB-R6_S4_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R6_S4_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12545149
Sequences flagged as poor quality0
Sequence length35-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1844431.4702336337336448No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1223220.9750541822978748No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTT368300.2935796139208869No Hit
CCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTCAGGTTTAAATTTAT324220.258442526270513No Hit
GCCCTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACT294880.23505500014388034No Hit
GTTTAAATTTATCTTTTTTCTTCTAAAAGTATGTTTTTATCTTCTAATTT189400.1509746914923051No Hit
CTCATTTTTTCAGTGCTATTTTATCCAATTTTTGGCTTTATATTTTTCTA159640.1272523746031235No Hit
CTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCC159020.12675815966793222No Hit
TTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTTC154340.12302763402810121No Hit
GCTTCCTACTTTTCAGGTTTAAATTTATCTTTTTTCTTCTAAAAGTATGT131840.10509241460583689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTT20900.031.1335531
CCCCTAT34350.015.4987991
CGGGCTA10550.014.5122111
CGCTAGT1951.488138E-414.2754151
TCGACCG5050.013.78644127
GCGGGTT11650.013.44063951
CGCGAAT2808.361674E-613.38914969
GCGACGT10250.013.24353722
CCGTTAA17200.013.15323521
GTCGCAT4407.548806E-1012.6532081
GCCGTAC4154.278263E-912.5769851
CGCTTGT18000.012.5653391
GCCGTTA16750.012.46728820
ACGGTTA16800.012.2198943
GCCCTAT44950.012.1535551
GTCGCTA11200.012.12160427
ATTCGGT13800.012.1030691
ACCGGTA3751.9637991E-712.06258210
GCCCTTA214900.012.0305591
CCCTTAG219750.011.859762
Produced by FastQC (version 0.11.5)