FastQCFastQC Report
lun 21 ene 2019
JVB-R5_S3_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R5_S3_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12208900
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT802420.6572418481599489No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA369640.30276273865786435No Hit
CTTAGCTTTTTGTTTCCTAGCTTGTCTTTTTTCTTCTGCTTCCTACTTTT126490.1036047473564367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTT15450.020.7104421
CGCGTAA10450.016.6411671
GCGTAAC11250.016.0757772
GCGACGT12850.015.97369222
TCGTTCG10950.015.57030528
CGCACTA2704.764115E-815.4577951
GTCGCTA14650.014.72396427
GCCGTTA20750.014.58576120
GTTATCG10150.014.40053234
CCGTTAA21500.014.23922621
CGCTAGT2708.01414E-714.1696451
ACGGTTA20450.014.1158143
GTATCGA9200.013.6093024
GTCCGAT5053.6379788E-1213.0855581
GGTACCG23100.012.94830611
CTATCGG10000.012.86993259
ATCGAAG26950.012.6471826
CGACGTC16550.012.6125423
ACTATCG11100.012.53447758
TATCGGA11850.012.33453136
Produced by FastQC (version 0.11.5)