FastQCFastQC Report
lun 21 ene 2019
JVB-R15_S15_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R15_S15_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14110297
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1322280.9371028830931056No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA467520.33133250136407477No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN317670.2251334610462133No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA237890.16859319119930644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAA18100.016.71718821
GCCGTTA18150.016.09598420
GACCGCA106200.016.0126119
GTGAAAG139100.015.1974041
ACCGCAA116100.014.798223520
CGCAATA117550.014.79409722
GGACCGC117150.014.4848418
CCGCAAT123500.014.10890721
AGGACCG123900.013.97576717
GCAATAG127400.013.650331523
TAGGTCC125300.013.60708533
CAATAGG132650.013.29376424
CGCGTAT2102.8082432E-413.2453571
AATAGGG136950.013.23215825
TACCGCG24950.013.0990413
AAGGGTC131600.013.00098450
GGTACCG23350.012.95431811
GAAAGAT149550.012.9493943
ATAGGGG142800.012.64141426
TGAAAGA157450.012.5867272
Produced by FastQC (version 0.11.5)