FastQCFastQC Report
lun 21 ene 2019
JVB-R9_S5_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R9_S5_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13772699
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT512630.37220736472930976No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA142430.10341473374245673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAT7100.014.2050781
ATAGACC43850.013.24958523
CGACCGT10950.013.02916828
TCGACCG10900.012.76825327
TAGACCA46800.012.41463424
GTTCGAC11400.012.20779925
GTATCCG21050.011.52612670
CGGGCTA9400.011.4693251
GCGGGTT15500.011.2186941
GGTCTAT52150.011.07033912
AGGTCTA54500.011.03972711
GGCGGTT16700.010.8290621
CGGGATA5251.2189048E-810.5989941
ACGGTTA13200.010.5385823
GTCGCTA10900.010.5338127
AAGTTCG14350.010.42490223
GACCATC58100.010.30023626
CATAGAC57500.010.22510922
ATCGCGT3803.6232275E-510.06730113
CGCGTAA10150.09.9365571
Produced by FastQC (version 0.11.5)