FastQCFastQC Report
lun 21 ene 2019
JVB-R8_S11_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R8_S11_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13208119
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435300.3295700167450036No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN163770.12399191739565642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1250.001841472316.692261
GTCGCTA10000.012.52422827
CCGGCAT15850.012.5060311
GACCGCA35700.012.47062219
GCGATCG11700.011.89642441
ACGTTCG12450.011.7313756
TCGCTAT11850.011.15719128
TAACGGG13050.011.14560169
CGCAATA42550.010.95525322
CGCTATG11850.010.86403429
ACCGCAA41400.010.83840520
GTCCGAT9400.010.3586731
CGCGCAT3753.134164E-510.2008261
GTCGTTT8900.010.1591471
CGTTCGA14550.010.0381867
CGATCGG16900.09.883183542
ATTGAGT41400.09.6597636
TTGCGAT6803.654588E-69.61237370
GCGGGTT14150.09.5847791
GCCGGTT17850.09.5462281
Produced by FastQC (version 0.11.5)