FastQCFastQC Report
lun 21 ene 2019
JVB-R7_S10_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R7_S10_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14288211
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412020.2883636026931573No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN147890.10350491044680121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG9000.015.07410827
CGACCGT9100.014.91028828
GTTCGAC10000.013.56660325
GTATCGA11450.013.3622224
TATCGAA13700.011.4215085
CCCGTAT20200.011.1893311
CGTATCG20850.011.007013
CGCTAAT3803.0309166E-610.9809471
ATGCGTA10800.010.94901157
GCCGTTA13450.010.60570629
CGCGTAA11500.010.5830861
ACGGTTA13850.010.54469612
ATCGCGC14400.010.44470867
CCGTTAA14050.010.40111630
GCGCTAT4056.4934648E-610.303111
ATCGCGT5401.8942956E-810.30293112
GTATACG15000.010.19990112
ATCGAAG33700.010.1118156
CTATCGG7951.8189894E-1210.06268559
AAGTTCG14600.010.00669723
Produced by FastQC (version 0.11.5)