FastQCFastQC Report
lun 21 ene 2019
JVB-R4_S9_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R4_S9_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16327076
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT606830.3716709593316035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA176480.10809038924054742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA3951.364242E-1014.0890654
GCCGTTA10900.013.72539720
AGTATTG38200.013.71554970
ACGGTTA10750.013.2658373
GTCGCTA11100.013.16614327
GTTCGAC10100.013.09181325
GGCGGTT18650.013.0552471
CGACCGT9900.012.65480428
GGCGGTC84150.012.5116870
TCGACCG10050.012.46432227
GCGATCG13200.012.12811641
CGCACTA3205.389764E-611.9566251
CCGTTAA12100.011.78926721
TTCGCAC30750.011.53767510
GCTGTAT45750.011.2521761
TCGGTAT10550.011.2094862
GTATTGA57700.011.0314994
TGTTACG6007.094059E-1111.014423
GTCGGAT13750.010.8775641
CGATCGG17050.010.8183842
Produced by FastQC (version 0.11.5)