FastQCFastQC Report
lun 21 ene 2019
JVB-R3_S8_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R3_S8_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15951224
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620860.3892240495149463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA263450.16515973946576137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG17200.016.38418427
CGACCGT17850.015.78993728
GTCGCAT5250.015.23632051
GTTCGAC18300.015.2091325
ATAGACC72100.014.08927223
GTCGCTA17250.013.71475527
TAGACCA73300.013.62143824
GCGACGT17550.013.47950922
AGGTCTA80000.012.65058111
GCCGATT5501.8189894E-1212.6467491
AAGTTCG22650.012.59466523
CGGGATA5255.456968E-1212.5865261
TCGCTAT19750.012.50906628
GGTCTAT82600.012.083961512
CGCTATG21200.011.81821929
CATAGAC86100.011.7175622
CGCGATT3003.6701706E-511.5928521
TTACGAC10000.011.4767085
GACCATC88400.011.45258426
GACCGTC26250.011.40051729
Produced by FastQC (version 0.11.5)