FastQCFastQC Report
lun 21 ene 2019
JVB-R2_S2_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R2_S2_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14646521
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1843421.258606053956431No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA989040.6752729880358619No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAA337760.230607664441269No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTG160740.10974619843169583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTT17100.022.373911
GACCGCA146450.017.7911719
CCGCAAT155750.016.84206821
ACCGCAA156000.016.72547320
GTGAAAG175500.016.3105281
GGACCGC163850.016.11255318
CGCAATA166050.015.88187422
TAGGTCC166000.015.78866633
GCAATAG168500.015.5479823
CAATAGG170050.015.42708924
ATGAGCT177050.015.2640728
AGGACCG178550.015.09718917
GAGGACC176750.014.99511416
TCGACCG12300.014.99334827
CGGGATA5850.014.8637871
TGAAAGA183300.014.667512
GATGAGC184600.014.5832097
ATAGGGG186600.014.41417526
AATAGGG190700.014.24990225
GTCGCTA22250.014.23112527
Produced by FastQC (version 0.11.5)