FastQCFastQC Report
lun 21 ene 2019
JVB-R14_S14_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R14_S14_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17478104
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357350.20445581511587296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAA16100.013.61006721
ACGGTTA15600.013.596833
GCCGTTA16800.012.83576620
ATCGCGT9900.012.00895967
GACCGTT24700.011.40692326
CGCACTA5251.020453E-911.2596731
ACCGTTC26050.011.21665127
GTCGCAA4854.3351974E-810.7543891
CGTATCG18500.010.5256453
CGCTATA3652.3289225E-510.479391
CGCGATA3004.7205278E-410.4317571
CGATCGG12800.010.32888942
GAGACCG28350.010.18352324
TTCGCAC26600.010.06564310
ACGCAAA25200.09.9349496
GTATCGA11550.09.934924
GTTCGAC10000.09.73951325
GCCGATT10400.09.6961841
CGACCGT9950.09.44046328
CGCAAAT7001.1878001E-99.4382551
Produced by FastQC (version 0.11.5)