FastQCFastQC Report
lun 21 ene 2019
JVB-R13_S13_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R13_S13_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15007205
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229300.1527932749635925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACT5900.016.504041
GTTACGA6600.014.7531694
AGTATTG36750.014.4296370
GTTCGAC11000.014.23239125
CGACCGT11150.014.04265728
TTCGCAC26750.013.3902610
TCGACCG11750.013.32389527
CGCGTAT1900.001987761812.81234551
ATCGCGT4351.116714E-711.19213413
ACTATCG5005.673428E-911.13110958
TGTTACG6301.4551915E-1111.0397863
TTTCGCA36200.010.7593249
TTACGAC6857.2759576E-1210.6610495
CTATCGG5653.4106051E-910.46644359
CGCGGTA6704.9112714E-1110.3810051
TCGCACC35900.010.26802611
GAGCGAC13450.010.08600657
GAGTATT38800.010.08176869
GTCGCAA5201.2009878E-710.0316451
CGTGCGA10550.09.89614238
Produced by FastQC (version 0.11.5)