FastQCFastQC Report
lun 21 ene 2019
JVB-R10_S12_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVB-R10_S12_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15072755
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT646700.42905228672528684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA198200.13149553615115486No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN175600.1165015950965832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATTG45450.014.93197870
GTTCGAC5950.014.61598225
ACGGTTA15050.014.0936123
ACGCTAG13450.013.97259465
TTCGCAC33500.013.80516210
CCGTTAA16100.013.61016521
GCCGTTA15850.013.60498620
CGACCGT6250.013.36001428
TATACGG16450.013.31704613
ATAGACC40950.012.74133323
GACCGTT16750.012.66855826
TCGACCG6700.012.46108327
GTATACG18350.012.31724212
GTCGCAT5206.730261E-1112.0370811
TTTCGCA42100.011.8936489
GAGTATT45100.011.77493569
TAGACCA43600.011.64803224
GGTCTAT45600.011.05705112
CACTCGG112650.011.01491970
CACGCTA17500.010.93602464
Produced by FastQC (version 0.11.5)